Journal: Translational Cancer Research

Article Title: BACE2 facilitates lung adenocarcinoma progression by enhancing mTORC1 signalling

doi: 10.21037/tcr-2025-1670

Figure Lengend Snippet: BACE2 positively regulates the mTORC1 signaling pathway. (A,B) GSEA indicated that 曹 is positively associated with the activation of the mTORC1 signalling pathway. (C,D) Western blot analysis revealed the expression levels of p-mTOR, total mTOR, p-RPS6KB1, and total RPS6KB1 in LUAD cells (GAPDH as loading control; n=3, non-parametric Mann-Whitney U test). *, P<0.05; **, P<0.01. BACE2, beta-secretase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GSEA, gene set enrichment analysis; LUAD, lung adenocarcinoma; mTORC1, mammalian target of rapamycin complex 1; p-RPS6KB1, phosphorylated ribosomal protein S6 kinase B1; p-mTOR, phosphorylated mammalian target of rapamycin; si-NC, small interfering negative control.

Article Snippet: Membranes were blocked with milk for 1 hour at 37 °C, incubated overnight at 4 °C with the following primary antibodies: p-mTOR (Proteintech, Wuhan, China; 67778-1-Ig, 1:10,000), mTOR (Abclonal, Wuhan, China; A24743, 1:1,000), phosphorylated ribosomal protein S6 kinase B1 (p-RPS6KB1, Abmart, Shanghai, China; PC5673, 1:300), RPS6KB1 (IPODIX, Wuhan, China; IPDX21973, 1:1,000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Proteintech, 60004-1-Ig).

Techniques: Activation Assay, Western Blot, Expressing, Control, MANN-WHITNEY, Negative Control

Journal: Translational Cancer Research

Article Title: BACE2 facilitates lung adenocarcinoma progression by enhancing mTORC1 signalling

doi: 10.21037/tcr-2025-1670

Figure Lengend Snippet: Blocking mTORC1 signaling reverses the impact of BACE2 overexpression on LUAD cells. (A) Western blot results showing the expression of BACE2, p-mTOR/mTOR, and p-RPS6KB1/RPS6KB1 following treatment with rapamycin (Rap) (GAPDH as loading control). (B,C) Cell viability and clonogenic growth (crystal violet staining) were assessed to evaluate proliferation. (D) Flow cytometry was used to analyse apoptosis. (E) Cell migration ability was assessed via Transwell assay (scale bar =50 µm, crystal violet staining, ×200). (F) Flow cytometric analysis of cell cycle distribution. *, P<0.05; **, P<0.01; ***, P<0.001 (non-parametric Mann-Whitney U test). BACE2, beta-secretase 2; CV, coefficient of variation; DJF, DNA Justified Fit; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mTOR, mammalian target of rapamycin; mTORC1, mammalian target of rapamycin complex 1; NC, negative control; OE, overexpression; p-RPS6KB1, phosphorylated ribosomal protein S6 kinase B1; p-mTOR, phosphorylated mammalian target of rapamycin; RMS, root mean square.

Article Snippet: Membranes were blocked with milk for 1 hour at 37 °C, incubated overnight at 4 °C with the following primary antibodies: p-mTOR (Proteintech, Wuhan, China; 67778-1-Ig, 1:10,000), mTOR (Abclonal, Wuhan, China; A24743, 1:1,000), phosphorylated ribosomal protein S6 kinase B1 (p-RPS6KB1, Abmart, Shanghai, China; PC5673, 1:300), RPS6KB1 (IPODIX, Wuhan, China; IPDX21973, 1:1,000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Proteintech, 60004-1-Ig).

Techniques: Blocking Assay, Over Expression, Western Blot, Expressing, Control, Staining, Flow Cytometry, Migration, Transwell Assay, MANN-WHITNEY, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Garlic-Derived S-Allylmercaptocysteine Ameliorates Nonalcoholic Fatty Liver Disease in a Rat Model through Inhibition of Apoptosis and Enhancing Autophagy

doi: 10.1155/2013/642920

Figure Lengend Snippet: Addition of SAMC reduced NAFLD-induced hepatic apoptosis via modulating the LKB1/AMPK and PI3 K/Akt pathways. Protein expressions of phosphorylated and total (a) LKB1, (b) AMPK, (c) PI3 K, and (d) Akt were measured by Western blot and then quantified by ImageJ software. Data presented are expressed as mean ± SEM ( n = 7), and experimental groups marked by different letters represented significant differences between groups at P < 0.05 (Kruskal-Wallis test followed by Dunn's post hoc test). N + S: NAFLD + SAMC cotreatment.

Article Snippet: Antibodies of phosphorylated liver kinase B1 (LKB1) at Ser428, total LKB1, phosphorylated AMP-activated protein kinase (AMPK) at Thr172, total AMPK, phosphorylated p53 at Ser15, total p53, phosphorylated Akt at Ser473, total Akt, total PI3 K (p85 subunit), cytochrome c, TNF-related apoptosis-inducing ligand (TRAIL), Fas, Fas-associated protein with death domain (FADD), cleaved caspase-3, cleaved caspase-8, phosphorylated mTOR at Ser2448, mTOR, beclin 1, Atg12, LC3 II, and p62 were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Software